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Key Documents

MAB5262

Sigma-Aldrich

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON®, mouse monoclonal, RT97

Synonim(y):

Anti-CMT2CC, Anti-NFH

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Neurofilament 200 kDa Antibody, clone RT97, clone RT97, Chemicon®, from mouse

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

RT97, monoclonal

reaktywność gatunkowa

mouse, human

reaktywność gatunkowa (przewidywana na podstawie homologii)

rat

producent / nazwa handlowa

Chemicon®

metody

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG1

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

Opis ogólny

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.

Specyficzność

The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).

Immunogen

Rat brain protein preparation

Zastosowanie

Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.
Immunohistochemistry:
1-10 μg/mL

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Jakość

Evaluated by Western Blot on Mouse brain membrane lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.

Opis wartości docelowych

200 kDa

Postać fizyczna

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8ºC from date of receipt.

Komentarz do analizy

Control
Brain

Cultured neurons

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A comparative study of a silver stain and monoclonal antibody reactions on Alzheimer's neurofibrillary tangles.
Connolly, A A, et al.
Journal of Neurology, Neurosurgery, and Psychiatry, 50, 1221-1224 (1987)
One year survival and significant reversal of motor deficits in parkinsonian rats transplanted with hESC derived dopaminergic neurons.
Ravindran Geeta,R L Ramnath,Harinarayana S Rao,Viswanathan Chandra
Biochemical and biophysical research communications null
Netrin-1 is a novel myelin-associated inhibitor to axon growth.
Low, K; Culbertson, M; Bradke, F; Tessier-Lavigne, M; Tuszynski, MH
The Journal of Neuroscience null
Normalization of striatal tyrosine hydroxylase and reversal of motor impairment in experimental parkinsonism with intravenous nonviral gene therapy and a brain-specific promoter.
Yun Zhang, Felix Schlachetzki, Yu-Feng Zhang, Ruben J Boado, William M Pardridge
Human Gene Therapy null
Alzheimer's disease: immunoreactivity of neurofibrillary tangles with anti-neurofilament and anti-paired helical filament antibodies.
Rasool, C G, et al.
Brain Research, 310, 249-260 (1984)

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