MAB349
Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5
clone 1.D5, Chemicon®, from mouse
Synonim(y):
Alz-90
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
1.D5, monoclonal
reaktywność gatunkowa
human, rat
producent / nazwa handlowa
Chemicon®
metody
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... APP(351)
Specyficzność
Reacts with the natural amyloid precursor protein A4(695).
Immunogen
Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].
Zastosowanie
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Postać fizyczna
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Przechowywanie i stabilność
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.
Maya Tanaka,Tatsuya Abe,Yoshitaka Hara
Journal of Cellular Physiology null
Axonal injury in young pediatric head trauma: a comparison study of ?-amyloid precursor protein (?-APP) immunohistochemical staining in traumatic and nontraumatic deaths.
Johnson, MW; Stoll, L; Rubio, A; Troncoso, J; Pletnikova, O; Fowler, DR; Li, L
Journal of Forensic Sciences null
Magdolna Pákáski et al.
Neurochemistry international, 47(3), 190-195 (2005-06-16)
Comorbid depression of Alzheimer's disease (AD) is a common mood disorder in the elderly and a broad spectrum of antidepressants have been used for its treatment. Abeta peptides and other derivatives of the amyloid precursor protein (APP) have been implicated
Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.
Weidemann, A, et al.
Cell, 57, 115-126 (1989)
3,4-Methylenedioxymethamphetamine (MDMA), but not morphine, alters APP processing in the rat brain.
Kalman, Janos, et al.
The International Journal of Neuropsychopharmacology, 10, 183-190 (2007)
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