MAB3140
Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9
clone 4E9, Chemicon®, from mouse
Synonim(y):
NHE1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
4E9, monoclonal
application:
WB
reaktywność gatunkowa:
amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)
metody:
western blot: suitable
citations:
27
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
4E9, monoclonal
reaktywność gatunkowa
amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)
reaktywność gatunkowa (przewidywana na podstawie homologii)
mouse, mammals, rat
producent / nazwa handlowa
Chemicon®
metody
western blot: suitable
izotyp
IgG1
przydatność
not suitable for immunoprecipitation
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SLC9A1(6548)
Opis ogólny
Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.
Specyficzność
Na+/H+ exchanger, isoform NHE1
Immunogen
Epitope: C-terminus
MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.
Zastosowanie
Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
Not recommended for immunoprecipitation.
Immunoblotting:
1:1,000 dilution used on a previous lot.
Optimal working dilutions must be determined by the user.
Jakość
Routinely evaluated by Western Blot on Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
Opis wartości docelowych
~100-110 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt.
Komentarz do analizy
Control
Kidney tissue (basolateral membrane)
Kidney tissue (basolateral membrane)
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Silencing of sodium/hydrogen exchanger in the heart by direct injection of naked siRNA.
Morgan, PE; Correa, MV; Ennis, IL; Ennis, IE; Diez, AA; Perez, NG; Cingolani, HE
Journal of Applied Physiology (1985)
The spatial organization of proton and lactate transport in a rat brain tumor.
Grillon, E; Farion, R; Fablet, K; De Waard, M; Tse, CM; Donowitz, M; Remy, C; Coles, JA
Testing null
Na(V)1.5 enhances breast cancer cell invasiveness by increasing NHE1-dependent H(+) efflux in caveolae.
Brisson, L, et al.
Oncogene (2010)
A novel chimeric natriuretic peptide reduces cardiomyocyte hypertrophy through the NHE-1-calcineurin pathway.
Kilic, Ana, et al.
Cardiovascular Research, 88, 434-442 (2010)
Stimulation of renal sulfate secretion by metabolic acidosis requires Na+/H+ exchange induction and carbonic anhydrase.
Pelis, RM; Edwards, SL; Kunigelis, SC; Claiborne, JB; Renfro, JL
American Journal of Physiology: Renal Physiology null
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