MAB2150
Anti-E-Selectin Antibody, clone P2H3
clone P2H3, Chemicon®, from mouse
Synonim(y):
ELAM-1, CD62E
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
pochodzenie biologiczne
mouse
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
P2H3, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SELE(6401)
Opis ogólny
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Specyficzność
Human E-Selectin
Immunogen
IL-1(1 activated HUVEC (human)
Zastosowanie
Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Postać fizyczna
Format: Purified
Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3.
Przechowywanie i stabilność
Maintain at 2-8°C for up to 12 months from date of receipt..
Komentarz do analizy
Control
Ductal breast cancer or mormal colon
Ductal breast cancer or mormal colon
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
H E Young et al.
The Anatomical record, 264(1), 51-62 (2001-08-16)
This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric
Preparation of chitosan films using different neutralizing solutions to improve endothelial cell compatibility.
Qing He,Qiang Ao,Yandao Gong,Xiufang Zhang
Journal of Materials Science. Materials in Medicine null
Natalya V Narizhneva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(9), 1158-1160 (2005-04-19)
Expression of cell adhesion molecules (CAM) responsible for leukocyte-endothelium interactions plays a crucial role in inflammation and atherogenesis. Up-regulation of vascular CAM-1 (VCAM-1), intracellular CAM-1 (ICAM-1), and E-selectin expression promotes monocyte recruitment to sites of injury and is considered to
Enhanced expression of alpha(1,3)-fucosyltransferase genes correlates with E-selectin-mediated adhesion and metastatic potential of human lung adenocarcinoma cells.
Martin-Satue, M, et al.
Cancer Research, 58, 1544-1550 (1998)
Preparation and characterization of chitosan-heparin composite matrices for blood contacting tissue engineering.
He Q, Ao Q, Gong K, Zhang L, Hu M, Gong Y, Zhang X
Biomedical Materials (Bristol, England) null
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej