MAB2030
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123
ascites fluid, clone BE-123, Chemicon®
Synonim(y):
Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
BE-123, monoclonal
reaktywność gatunkowa
human, canine, rat, sheep, bovine, mouse
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CSPG4(1464)
Specyficzność
Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.
Immunogen
Mouse proteoglycan.
Zastosowanie
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
ELISA at 1:400-1:3,200.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Postać fizyczna
Ascites fluid containing no preservatives.
Przechowywanie i stabilność
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
A human schwannoma cell line supports the in vitro adhesion of Plasmodium falciparum infected erythrocytes to chondroitin-4-sulfate.
K T Andrews, N K Viebig, F Wissing, N Klatt, N Oster, H Wickert, P Knolle, M Lanzer
Parasitology Research null
Cytoadherence of Plasmodium falciparum-infected erythrocytes in the human placenta.
B Maubert, N Fievet, G Tami, C Boudin, P Deloron
Parasite Immunology null
Effect of cytokine treatment on the in vitro expression of the P. falciparum adhesion receptor chondroitin-4-sulphate on the surface of human choriocarcinoma (BeWo) cells.
Yvonne Adams,Reinhard Schwartz-Albiez,James S McCarthy,Katherine T Andrews
Parasitology Research null
Efficient secretion of biologically active Chondroitinase ABC from mammalian cells in the absence of an N-terminal signal peptide.
Michael Kluppel
Molecular and Cellular Biochemistry null
Adherence of Plasmodium falciparum infected erythrocytes to CHO-745 cells and inhibition of binding by protein A in the presence of human serum.
K T Andrews, Y Adams, N K Viebig, M Lanzer, R Schwartz-Albiez
International Journal For Parasitology null
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