MAB1614
Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
clone H2, Chemicon®, from mouse
Synonim(y):
Anti-HEL-S-61, Anti-KINH, Anti-KNS, Anti-KNS1, Anti-UKHC
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
H2, monoclonal
reaktywność gatunkowa
sea urchin, rat, chicken, squid, human, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
mammals
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
izotyp
IgG2b
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... KIF5B(3799)
Opis ogólny
Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
Specyficzność
Reacts with the heavy chain of kinesin near the N-terminus.
Immunogen
Bovine brain kinesin.
Epitope: a.a.420-445
Zastosowanie
Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Jakość
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Opis wartości docelowych
124 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Komentarz do analizy
Control
Brain tissue
Brain tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.
Brady, S T, et al.
Proceedings of the National Academy of Sciences of the USA, 87, 1061-1065 (1990)
Conventional kinesin holoenzymes are composed of heavy and light chain homodimers.
DeBoer, Scott R, et al.
Biochemistry, 47, 4535-4543 (2008)
T Lynne Blasius et al.
PloS one, 8(9), e76081-e76081 (2013-10-08)
Kinesin motors drive the long-distance anterograde transport of cellular components along microtubule tracks. Kinesin-dependent transport plays a critical role in neurogenesis and neuronal function due to the large distance separating the soma and nerve terminal. The fate of kinesin motors
c-Jun NH2-terminal kinase-interacting protein-3 facilitates phosphorylation and controls localization of amyloid-beta precursor protein.
Muresan, Z; Muresan, V
The Journal of Neuroscience null
Processive bidirectional motion of dynein-dynactin complexes in vitro.
Jennifer L Ross, Karen Wallace, Henry Shuman, Yale E Goldman, Erika L F Holzbaur
Nature Cell Biology null
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