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Key Documents

MAB1430

Sigma-Aldrich

Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12)

clone 24.12.8 (PHM-12), Chemicon®, from mouse

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

klon

24.12.8 (PHM-12), monoclonal

reaktywność gatunkowa

human

producent / nazwa handlowa

Chemicon®

metody

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

izotyp

IgG1

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

BIOCHEMISTRY:

p I = 5.5

Specyficzność

MAB1430 provides a marker for an antigen known to be important in glomerular cell-matrix interactions, and should prove useful in research into the mechanisms involved in renal pathology. This antibody reacts with glomerular and tubular basement membranes in kidney as well as basal lamina of capillaries. It also labels mesangial cells and matrix within the glomerulus as well as basement membrane structures in all organs examined (Gusterson et al., 1984).

Immunogen

Human glomeruli

Zastosowanie

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12) validated for use in IH, IH(P).
The antibody is suitable for use on both frozen and protease digested paraffin processed tissue.

SUGGESTED USAGE

Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.

Optimal working dilutions must be determined by the end user.

Postać fizyczna

Format: Purified
Purified immunoglobulin presented as a liquid in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide.

Przechowywanie i stabilność

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Diffusible factors released by fibroblasts support epidermal morphogenesis and deposition of basement membrane components.
El Ghalbzouri, Abdoelwaheb and Ponec, Maria
Wound Repair and Regeneration, 12, 359-367 (2004)
Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis.
Davis, LS; Sackler, M; Brezinschek, RI; Lightfoot, E; Bailey, JL; Oppenheimer-Marks, N; Lipsky, PE
The American Journal of Pathology null
Invading squamous cell carcinoma can retain a basal lamina. An immunohistochemical study using a monoclonal antibody to type IV collagen.
Gusterson, B A, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 51, 82-87 (1984)
Production of monoclonal antibodies to fibronectin, type IV collagen and other antigens of the human glomerulus.
Hancock, W W, et al.
Pathology, 16, 197-206 (1984)
S E Aggrey et al.
Poultry science, 73(12), 1822-1828 (1994-12-01)
Records of 1,530 Japanese quail were used to estimate heritabilities and genetic correlations based on a derivative-free restricted maximum likelihood (REML) method with an animal model and ANOVA. The animal model included fixed effects of hatch and sex, random effects

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