MAB1398Z
Anti-PECAM-1 Antibody, clone 2H8, Azide Free
clone 2H8, Chemicon®, from hamster(Armenian)
Synonim(y):
CD31, PECAM-1, Platelet endothelial cell adhesion molecule
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
hamster (Armenian)
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
2H8, monoclonal
reaktywność gatunkowa
mouse, canine
producent / nazwa handlowa
Chemicon®
metody
electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
dog ... Pecam1(610775)
mouse ... Pecam1(18613)
Powiązane kategorie
Opis ogólny
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Specyficzność
React s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation.
Immunogen
D10.G4.1 murine T cells (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843 -854).
Epitope: Extracellular domain.
Zastosowanie
Anti-PECAM-1 Antibody, clone 2H8, Azide Free detects level of PECAM-1 & has been published & validated for use in Elecron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Neutralization Assay.
Immunofluorescence Analysis: Representative lots immunostained endothelial blood vessel cells in 4% paraformaldehyde-fixed frozen tumor sections from mice and dogs as a means for assessing lymph vessel density in tumor xenografts by fluorescent immunohistochemistry (Donat, U., et al. (2012). PLoS One. 7(9):e45942; Gentschev, I., et al. (2012). PLoS One.7(5):e37239).
Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Powiązanie
Replaces: 04-1074
Postać fizyczna
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.
Protein A purified
Przechowywanie i stabilność
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
Mouse cell lung tissue
Mouse cell lung tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Developmental Dynamics null
Activation-dependent changes in human platelet PECAM-1: phosphorylation, cytoskeletal association, and surface membrane redistribution.
Newman, P J, et al.
The Journal of cell biology, 119, 239-246 (1992)
S Bogen et al.
The Journal of experimental medicine, 179(3), 1059-1064 (1994-03-01)
A murine model of peritonitis was used to test the role of platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31) in acute inflammation. A monoclonal antibody (mAb) specific for murine PECAM-1 injected intravenously 4 h before the intraperitoneal injection of thioglycollate broth
Produkty
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