GF35L
Anti-BDNF Mouse mAb (35928.11)
lyophilized, clone 35928.11, Calbiochem®
Synonim(y):
Anti-Brain Derived Neurotrophic Factor
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
35928.11, monoclonal
Postać
lyophilized
nie zawiera
preservative
reaktywność gatunkowa
human
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG1
Warunki transportu
ambient
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... BDNF(627)
Opis ogólny
Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.
Immunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Immunogen
recombinant protein consisting of amino acids 129-247 of human BDNF
Zastosowanie
ELISA (0.5-1 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
Lyophilized from PBS, 5% trehalose.
Rekonstytucja
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Inne uwagi
Riccio, A., et al. 1997. Science277 1097.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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