FC010

Human Plasma Fibronectin Purified Protein

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

• Kod UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Właściwości

• pochodzenie biologiczne: human
• Poziom jakości: 100; 300
• sterylność: sterile; sterile-filtered
• Próba: ~95% (SDS-PAGE)
• Postać: liquid
• masa cząsteczkowa: 220 kDa
• producent / nazwa handlowa: Chemicon^®
• stężenie: 1 mg/mL
• metody: cell culture | mammalian: suitable
• pokrycie powierzchni: 2—10 μg/cm²
• moc wejściowa: sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type: human embryonic stem cell(s); sample type epithelial cells; sample type hematopoietic stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s)
• numer dostępu NCBI: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• numer dostępu UniProt: P02751
• Specyficzność wiązania: Peptide Source: Collagen; ; Peptide Source: Laminin
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• informacje o genach: human ... FN1(2335)

Opis

Opis ogólny

Fibronectins (FNs) are high-molecular-mass adhesive glycoproteins of the extracellular matrix (ECM) and body fluids. It is a dimer composed of two subunits linked by two disulfide bonds. Each monomer of FN consists of three types of repeating units: 12 FN repeats of type I (approximately 40 amino acids each), two type II repeats (around 60 amino acids), and 15-17 type III repeats (approximately 90 amino acids). These repeating units contribute to the structural and functional diversity of fibronectin molecules.

Zastosowanie

Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

Działania biochem./fizjol.

Fibronectin plays a crucial role in mediating diverse cellular interactions and is involved in various processes, including cell adhesion, the establishment and maintenance of cell morphology, cell migration, growth, and differentiation. It interacts with several extracellular matrix (ECM) and cell surface proteins, such as collagen, heparin, fibrin, and specific cell membrane receptors. Fibronectin can also serve as a ligand for multiple integrins, including the well-known fibronectin receptor alpha5-beta1, which binds to the arginylglycylaspartic acid (RGD) sequence in the repeat III-10 region of fibronectin. This extensive network of interactions highlights fibronectin′s significance in regulating cellular behavior and mediating tissue function.

Postać fizyczna

Liquidin 50 mM tris-buffered saline (TBS), pH 7.5, sterile filtered, containing nopreservatives.

Przechowywanie i stabilność

Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.

Komentarz do analizy

A double band of 220 kDa is present under reduced conditions.hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.

Inne uwagi

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm²) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm².

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3