CP53

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

liquid, clone W-CAM-1, Calbiochem^®

• Synonim(y): Anti-CD54, Anti-Inter-Cellular Adhesion Molecule, Anti-Inter-Cellular Adhesion Molecule, Anti-CD54
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: W-CAM-1, monoclonal
• Postać: liquid
• zawiera: ≤0.1% sodium azide as preservative
• reaktywność gatunkowa: human
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• izotyp: IgG2b
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ICAM1(3383)

Opis

Opis ogólny

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.

Recognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Immunogen

Human

Raji cells

Zastosowanie

Cell Inhibition Assays (see comments)

Flow Cytometry (see comments)

Frozen Sections (1:50-1:100)

Immunoblotting (1:200-1:400)

Immunofluorescence (see comments)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (not recommended)

Opakowanie

Please refer to vial label for lot-specific concentration.

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

In 10 mM PBS, 0.2% BSA, pH 7.4.

Rekonstytucja

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Komentarz do analizy

Positive Control
Endothelial cells or tonsil tissue

Inne uwagi

Takami, S., et al. 1998. Circ.97, 721.
Craig, A.G., et al. 1997. Infect. Immun.65, 4580.
DeGraba, T.J. 1997. Neurology49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest.15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol.143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med.177, 1187.
Fawcett, J., et al. 1992. Nature360, 481.
Vazeux, R. et al. 1992. Nature360, 485.
Boyd, A.W., et al. 1989. Blood73, 1896.

This antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable