ABF131

Anti-phospho TIE2/TEK Antibody (Tyr992)

0.25 mg/mL, from rabbit

• Synonim(y): Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p140 TEK, CD202b
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology)
• stężenie: 0.25 mg/mL
• metody: immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• numer dostępu NCBI: NP_000450
• numer dostępu UniProt: Q02763
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pTyr992)
• informacje o genach: human ... TEK(7010)

Opis

Opis ogólny

TIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Immunogen

Epitope: Protein Kinase Domain

KLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.

Zastosowanie

Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).

Jakość

Evaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.

Opis wartości docelowych

~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Powiązanie

Replaces: 09-407

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable