ABE1788

Anti-Nicotinamide N-methyltransferase

from rabbit, purified by affinity chromatography

• Synonim(y): EC 2.1.1.1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μg
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NP_006160.1
• numer dostępu UniProt: P40261
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... NNMT(4837)

Opis

Opis ogólny

Nicotinamide N-methyltransferase (UniProt: P40261; also known as EC: 2.1.1.1) is encoded by the NNMT gene (Gene ID: 4837) in human. Nicotinamide N-methyltransferase is a monomeric, cytoplasmic enzyme that belong to the class I -like SAM-binding methyltransferase superfamily. It catalyzes the N-methylation of nicotinamide and other pyridines with S-Adenosyl-L-methionine (SAM)) serving as the methyl donor. It has two SAM binding sites (aa 63-64 and 142-143). Its catalytic activity is important for biotransformation of many drugs and xenobiotic compounds. Nicotinamide N-methyltransferase is predominantly expressed in the liver. A lower level of expression is seen in the kidney, lung, skeletal muscle, placenta, and heart and it is not detected in the brain or pancreas. Nicotinamide N-methyltransferase inhibition is shown to increases adipose SAM and NAD(+) levels and upregulate ornithine decarboxylase and spermidine-spermine N(1)-acetyltransferase activity and expression. (Ref.: Kraus, D et al. (2014). Nature. 508 (7495); 258-62).

Specyficzność

This rabbit polyclonal antibody detects human Nicotinamide N-methyltransferase. It targets an epitope within 15 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Nicotinamide N-methyltransferase.

Zastosowanie

Anti-Nicotinamide N-methyltransferase Antibody, Cat. No. ABE1788, is a highly specific rabbit polyclonal antibody that targets human Nicotinamide N-methyltransferase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 1 µg/mL from a representative lot detected Nicotinamide N-methyltransferase in 10 µg of human kidney lysate and HEK293 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nicotinamide N-methyltransferase in human liver, human kidney, and human pancreas tissues.

Jakość

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nicotinamide N-methyltransferase in 10 µg of HepG2 cell lysate.

Opis wartości docelowych

~30 kDa observed; 29.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash