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Key Documents

AB9076

Sigma-Aldrich

Anti-IP3 Receptor 3 Antibody

serum, Chemicon®

Synonim(y):

Inositol 1,4,5-Trisphosphate Receptor 3

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

serum

rodzaj przeciwciała

primary antibodies

klon

polyclonal

reaktywność gatunkowa

mouse, rat, human

producent / nazwa handlowa

Chemicon®

metody

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ITPR3(3710)
mouse ... Itpr3(16440)
rat ... Itpr3(25679)

Specyficzność

IP3 Receptor 3.

Immunogen

Synthetic peptide from the C-terminus of rat IP3 Receptor 3.

Zastosowanie

Anti-IP3 Receptor 3 Antibody detects level of IP3 Receptor 3 & has been published & validated for use in IC, IH & WB.
Research Category
Signaling
Research Sub Category
Lipid Signaling
Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 3 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Postać fizyczna

Rabbit serum. Liquid.

Przechowywanie i stabilność

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Komentarz do analizy

Control
Western blot = adult mouse pancreas.

IHC = adult mouse hepatocytes (adult mouse cerebellum granule cells will be negative).

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Laura Marongiu et al.
Science signaling, 14(676) (2021-04-01)
Innate immune responses to Gram-negative bacteria depend on the recognition of lipopolysaccharide (LPS) by a receptor complex that includes CD14 and TLR4. In dendritic cells (DCs), CD14 enhances the activation not only of TLR4 but also that of the NFAT
S Kaja et al.
Neuroscience, 175, 281-291 (2010-11-16)
Dysregulation of Ca(2+) signaling following oxidative stress is an important pathophysiological mechanism of many chronic neurodegenerative disorders, including Alzheimer's disease, age-related macular degeneration, glaucomatous and diabetic retinopathies. However, the underlying mechanisms of disturbed intracellular Ca(2+) signaling remain largely unknown. We
Function and expression of ryanodine receptors and inositol 1,4,5-trisphosphate receptors in smooth muscle cells of murine feed arteries and arterioles.
Westcott, EB; Goodwin, EL; Segal, SS; Jackson, WF
The Journal of Physiology null
Pancreatic protease activation by alcohol metabolite depends on Ca2+ release via acid store IP3 receptors.
Gerasimenko, JV; Lur, G; Sherwood, MW; Ebisui, E; Tepikin, AV; Mikoshiba, K; Gerasimenko et al.
Proceedings of the National Academy of Sciences of the USA null
Sofia Håglin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(21), 4116-4129 (2020-05-10)
The cellular and molecular basis of metaplasia and declining neurogenesis in the aging olfactory epithelium (OE) remains unknown. The horizontal basal cell (HBC) is a dormant tissue-specific stem cell presumed to only be forced into self-renewal and differentiation by injury.

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