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AB5818

Sigma-Aldrich

Anti-Potassium Channel Kir4.1 Antibody

Chemicon®, from rabbit

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

forma przeciwciała

affinity purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

rat, mouse, human

producent / nazwa handlowa

Chemicon®

metody

western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... KCNJ10(3766)

Specyficzność

Recognizes Kir4.1. The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Other species have not been tested.

Immunogen

CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Synthetic peptide corresponding to amino acids 356-375 of rat, mouse or human Kir4.1 (Accession P49655).

Zastosowanie

This Anti-Potassium Channel Kir4.1 Antibody is validated for use in WB for the detection of Potassium Channel Kir4.1.
Western blot: 1:200-1:600 using ECL on rat brain membranes or human platelets.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Postać fizyczna

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for

Komentarz do analizy

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 3

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3


Certyfikaty analizy (CoA)

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