AB5186
Anti-Potassium Channel Kv2.1 Antibody
Chemicon®, from rabbit
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... KCNB1(3745)
Specyficzność
Recognizes a full length Kv2.1 protein. Does not cross react with any other potassium channel antigens tested so far.
SPECIES REACTIVITIES: Should work in mouse (100% epitope homology) and in human and rabbit (88% epitope homology). Other species have not been tested.
SPECIES REACTIVITIES: Should work in mouse (100% epitope homology) and in human and rabbit (88% epitope homology). Other species have not been tested.
Immunogen
Purified peptide from rat Kv2.1 (amino acids 841-857) (Accession P15387),
Zastosowanie
Anti-Potassium Channel Kv2.1 Antibody detects level of Potassium Channel Kv2.1 & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1:250-1:500 using ECL on rat brain membranes.
Immunohistochemistry using rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry using rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Postać fizyczna
Format: Purified
Purified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Przechowywanie i stabilność
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Komentarz do analizy
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 50 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 50 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
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L Betancourt et al.
Journal of neuroscience research, 61(6), 646-651 (2000-09-06)
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Mark G MacAskill et al.
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