AB3403-I

Anti-Beta Galactosidase Antibody, E. coli

from chicken, purified by affinity chromatography

• Synonim(y): Beta-galactosidase, Acid beta-galactosidase, Lactase, Elastin receptor 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: chicken
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: bacteria
• metody: western blot: suitable
• numer dostępu NCBI: NP_752394
• numer dostępu UniProt: P16278
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: Escherichia coli K12 ... Lacz(945006)

Opis

Opis ogólny

β Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.

Immunogen

Recombinant protein corresponding to E. Coli Beta Galactosidase.

Zastosowanie

Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.

Jakość

Evaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.

Opis wartości docelowych

~120 kDa observed

Powiązanie

Replaces: AB3403

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable