AB1534SP
Anti-Brain Derived Neurotrophic Factor Antibody
Chemicon®, from rabbit
Synonim(y):
BDNF
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
mouse, rat, human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... BDNF(627)
Specyficzność
Recognizes Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.
Immunogen
A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin. The sequence is H2N-HSDPARRGEL-COOH.
Zastosowanie
Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, IH & WB.
Immunohistochemistry: 1-10 μg/mL (see suggested protocol).
Immunoblotting: 1-10 μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as 18 kDa, with a homodimer at ~27-30 kDa.
ELISA (one-site): 1-10 μg/mL
Optimal working dilutions must be determined by end user.
Immunoblotting: 1-10 μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as 18 kDa, with a homodimer at ~27-30 kDa.
ELISA (one-site): 1-10 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Opis wartości docelowych
18 kDa (monomer) and 27-30 kDa (homodimer)
Postać fizyczna
Format: Purified
Protein A Purified immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Protein A purified
Przechowywanie i stabilność
Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Komentarz do analizy
Control
Brain tissue
Brain tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Differential expression of neurotrophins in postnatal C57BL/6 mice striatum.
V Zerme?o,S Espindola,E Mendoza,E Hernandez-Echeagaray
International Journal of Biological Sciences null
Antonino Germanà et al.
International journal of molecular sciences, 21(16) (2020-08-19)
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB
Luciano Domenici et al.
PloS one, 9(12), e115579-e115579 (2014-12-24)
Vision loss in glaucoma is caused by progressive dysfunction of retinal ganglion cells (RGCs) and optic nerve atrophy. Here, we investigated the effectiveness of BDNF treatment to preserve vision in a glaucoma experimental model. As an established experimental model, we
Ewa Badowska-Szalewska et al.
Acta neurobiologiae experimentalis, 77(1), 68-76 (2017-04-06)
The way hippocampal neurons function during stress in old age (critical times of life) is dependent on brain derived neurotrophin factor (BDNF). This study examined the influence of acute and chronic forced swim (FS) or high-light open field (HL‑OF) stimulation
Yingying Zhou et al.
Frontiers in cellular neuroscience, 10, 307-307 (2017-01-26)
Post-partum depression (PPD) is a common mental disease in the perinatal period that profoundly affects mothers and their offspring. Some clinical studies have found that PPD is related to thyroid peroxidase antibodies (TPOAbs); however, the mechanism underlying this relationship is
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