AB10523
Anti-EBF-1 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Transcription factor COE1, O/E-1, OE-1, Early B-cell factor
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human, mouse
metody
dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... EBF1(1879)
Opis ogólny
The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF1 is abundantly expressed in early B-cells, lymph node, spleen, and adipose tissue. Low levels of EBF1 have also been detected in the brain, heart, skeletal muscle, and kidney. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5′-ATTCCCNNGGGAATT-3′. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.
Specyficzność
This antibody recognizes EBF-1 but not EBF-2 or EBF-3.
Immunogen
KLH-conjugated linear peptide corresponding to mouse EBF-1.
Zastosowanie
Anti-EBF-1 Antibody is an antibody against EBF-1 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blot Analysis: 2 µg/mL from a representative lot detected EBF-1 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-1 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-1 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-1 (1:100 dilution). No cross reactivity to peptides for EBF-3 & EBF-2 were observed.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-1 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-1 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-1 (1:100 dilution). No cross reactivity to peptides for EBF-3 & EBF-2 were observed.
Jakość
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected EBF-1 in 10 µg of Raji cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected EBF-1 in 10 µg of Raji cell lysate.
Opis wartości docelowych
~66 kDa observed. Uncharacterized bands may appear between ~40 and ~170 kDa in some lysates.
Postać fizyczna
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
Raji cell lysate
Raji cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Xiaolei Song et al.
Neuroscience bulletin, 37(7), 985-998 (2021-05-21)
Medium spiny neurons (MSNs) in the striatum, which can be divided into D1 and D2 MSNs, originate from the lateral ganglionic eminence (LGE). Previously, we reported that Six3 is a downstream target of Sp8/Sp9 in the transcriptional regulatory cascade of
Zhenmeiyu Li et al.
Cell death discovery, 8(1), 301-301 (2022-07-01)
The striatum is the main input structure of the basal ganglia, receiving information from the cortex and the thalamus and consisting of D1- and D2- medium spiny neurons (MSNs). D1-MSNs and D2-MSNs are essential for motor control and cognitive behaviors
Paloma Merchan-Sala et al.
The Journal of comparative neurology, 525(13), 2805-2819 (2017-05-05)
The striatum is the major component of the basal ganglia and is well known to play a key role in the control of motor function via balanced output from the indirect (iSPNs) and direct pathway striatal projection neurons (dSPNs). Little
Francesca Pagani et al.
Histochemistry and cell biology, 156(4), 333-347 (2021-07-18)
Early B-cell factor-1 (EBF1) is a transcription factor with an important role in cell lineage specification and commitment during the early stage of cell maturation. Originally described during B-cell maturation, EBF1 was subsequently identified as a crucial molecule for proper
Teng Guo et al.
Cerebral cortex (New York, N.Y. : 1991), 29(11), 4831-4849 (2019-02-24)
Generation of olfactory bulb (OB) interneurons requires neural stem/progenitor cell specification, proliferation, differentiation, and young interneuron migration and maturation. Here, we show that the homeobox transcription factors Dlx1/2 are central and essential components in the transcriptional code for generating OB
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