8.52095

Fmoc-Lys(Mca)-OH

≥97.0% (HPLC), for peptide synthesis, Novabiochem^®

• Synonim(y): Fmoc-Lys(Mca)-OH, N-α-Fmoc-N-ε-7-methoxycoumarin-4-acetyl-L-lysine
• Wzór empiryczny (zapis Hilla): C₃₃H₃₂N₂O₈
• Numer CAS: 386213-32-7
• Masa cząsteczkowa: 584.62
• Kod UNSPSC: 12352209
• NACRES: NA.22

Właściwości

• product name: Fmoc-Lys(Mca)-OH, Novabiochem^®
• Poziom jakości: 200
• linia produktu: Novabiochem^®
• Próba: ≥97% (TLC); ≥97.0% (HPLC)
• Postać: powder
• przydatność reakcji: reaction type: Fmoc solid-phase peptide synthesis
• producent / nazwa handlowa: Novabiochem^®
• Zastosowanie: peptide synthesis
• grupa funkcyjna: amine
• temp. przechowywania: 15-25°C
• InChI: 1S/C33H32N2O8/c1-41-21-13-14-22-20(17-31(37)43-29(22)18-21)16-30(36)34-15-7-6-12-28(32(38)39)35-33(40)42-19-27-25-10-4-2-8-23(25)24-9-3-5-11-26(24)27/h2-5,8-11,13-14,17-18,27-28H,6-7,12,15-16,19H2,1H3,(H,34,36)(H,35,40)(H,38,39)/t28-/m0/s1
• Klucz InChI: BWJJONDPLYEAMC-NDEPHWFRSA-N

Opis

Opis ogólny

A modified lysine derivative for the preparation of fluorogenically-labeled peptides by Fmoc SPPS ^[1]. The Mca group fluoresces at 405 nm when stimulated at 340 nm, and is most commonly used in conjunction with Dabcyl and 2,4-dinitrophenyl quenching groups. Recently, Fmoc-Lys(Mca)-OH has been used to prepare lanthanide-based luminescent proteins by native chemical ligation ^[2]. Lys(Mca) was found to be stable to HF cleavage conditions used to synthesize the thioester fragments needed for ligation.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] J. L. Lauer-Fields, et al. (2001) Biochemistry, 40, 5795.
^[2] C. F. W. Becker, et al. (2004) Bioconjugate Chem., 15, 1118.

Powiązanie

Replaces: 04-12-1233

Komentarz do analizy

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.0 % (a/a)
Purity (TLC(011A)): ≥ 97 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Water (K. F.): ≤ 2.0 %

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Informacje prawne

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable