524629
Phosphatase Inhibitor Cocktail V
50X, liquid, for the inhibition of serine/threonine and tyrosine phosphatase
Synonim(y):
Phosphatase Inhibitor Mix Set V, Phosphatase inhibitor
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.77
Polecane produkty
product name
Phosphatase Inhibitor Cocktail Set V, 50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
Postać
liquid
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
protect from light
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.
Zastosowanie
Phosphatase Inhibitor Cocktail Set V, 50X has been used:
- as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
- in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
- in lysis buffer to lyse cultured HUVECs for immunoprecipitation
- in wash buffer to wash the protein complexes
- as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity
Działania biochem./fizjol.
Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.
Cechy i korzyści
- Convenient: formulations are ready-to-use, making them easy to use.
- Consistent: high-quality formulations ensure reliable and reproducible performance.
- Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.
Komponenty
Each vial contains 1 ml of aqueous solution with the following components:
- 250 mM Sodium Fluoride
- 50 mM ß-Glycerophosphate (Cat. No. 35675)
- 50 mM Sodium Pyrophosphate Decahydrate
- 50 mM Sodium Orthovanadate
Ostrzeżenie
Toxicity: Toxic (F)
Postać fizyczna
In H₂O.
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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