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48-681MAG

Millipore

MILLIPLEX® Multi-Pathway Total Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay

Synonim(y):

Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Panel

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.47

reaktywność gatunkowa

human, mouse, rat

Poziom jakości

producent / nazwa handlowa

Milliplex®

metody

multiplexing: suitable

metoda wykrywania

fluorometric (Luminex® xMAP®)

temp. przechowywania

2-8°C

Opis ogólny

The MILLIPLEX® ColA MAP 9-plex Multi-Pathway Total Magnetic Bead kit is used to detect total protein levels for ERK/MAP kinase 1/2, Akt, STAT3, JNK, p70 S6 kinase, NFκB, STAT5A/B, CREB, and p38 in cell lysates using the Luminex® xMAP technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

Specyficzność

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Zastosowanie

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.Analytes available:Erk/MAPK 1/2 (Total);Akt (Total);STAT3 (Total);JNK (Total);p70 S6 Kinase (Total);NFκB (Total);STAT5A/B (Total);CREB (Total);p38 (Total)

Przechowywanie i stabilność

Recommended storage for kit components is 2 - 8°C

Informacje prawne

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Kod klasy składowania

10 - Combustible liquids


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James P McNamee et al.
International journal of radiation biology, 97(9), 1316-1323 (2021-05-29)
To assess the effects of 1800 MHz radiofrequency electromagnetic field (RF-EMF) exposure on the expression of signal transduction and antioxidant proteins in a human-derived A172 glioblastoma cell line. Adherent human-derived A172 glioblastoma cells (1.0 × 105 cells per 35 mm culture dish, containing 2 mL
Tania Quesada-López et al.
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The thermogenic activity of brown adipose tissue (BAT) and browning of white adipose tissue are important components of energy expenditure. Here we show that GPR120, a receptor for polyunsaturated fatty acids, promotes brown fat activation. Using RNA-seq to analyse mouse
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The Journal of biological chemistry, 298(3), 101675-101675 (2022-02-06)
A multienzyme metabolic assembly for human glucose metabolism, namely the glucosome, has been previously demonstrated to partition glucose flux between glycolysis and building block biosynthesis in an assembly size-dependent manner. Among three different sizes of glucosome assemblies, we have shown

Powiązane treści

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Testy sygnalizacji komórkowej MILLIPLEX® ułatwiają wykrywanie fosfoprotein i białek całkowitych w szlakach komórkowych.

See how MILLIPLEX® multiplex immunoassays offer researchers the ability to simultaneously quantitate a large number of human cytokines, chemokines, and growth factors, and detect human immunoglobulins against SARS-CoV-2 antigens, to better understand the immune response to COVID-19, including cytokine storm.

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