About This Item
Kod UNSPSC:
41116012
eCl@ss:
32160405
Polecane produkty
producent / nazwa handlowa
Chemicon®
metody
protein extraction: suitable
Zastosowanie
sample preparation
Warunki transportu
dry ice
temp. przechowywania
2-8°C
Zastosowanie
CHEMICON′s Whole Cell Extraction Kit (Catalog No. 2910) provides a simple and convenient method for the preparation of whole cell extractions for use in experimental studies of various cellular proteins.
This kit is recommended for use with CHEMICON′s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON′s Transcription Factor Assays when purified nuclear extracts are not required.
For Research Use Only; Not for use in diagnostic procedures
This kit is recommended for use with CHEMICON′s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON′s Transcription Factor Assays when purified nuclear extracts are not required.
For Research Use Only; Not for use in diagnostic procedures
Research Category
All
All
Komponenty
Whole Cell Extraction Buffer, 5x: - (Part No. 90493) Two vials containing 10 mL of 5x concentrated buffer. Dilute to 1x final concentration with ddH2O prior to use.
Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
Przechowywanie i stabilność
· The 5x Whole Cell Extraction Buffer can be stored at 2-8°C until expiration date.
· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.
· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.
· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Andrew J Schneider et al.
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Yuxuan Xiao et al.
Biochemical and biophysical research communications, 487(3), 640-645 (2017-04-25)
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes
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