17-643
ChIPAb+ Monomethyl-Histone H3 (Lys27) - przeciwciało i zestaw primerów zweryfikowane pod kątem ChIP
serum, from rabbit
Synonim(y):
H3K27me1, histon H3 (mono metyl K27)
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.52
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
klon
polyclonal
reaktywność gatunkowa
human, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
mammals
producent / nazwa handlowa
ChIPAb+
Upstate®
metody
ChIP: suitable
electrophoretic mobility shift assay: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
informacje o genach
human ... H3F3B(3021)
Opis ogólny
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Monomethyl-Histone H3 (Lys27) set includes the anti-monomethyl-histone H3 (Lys27) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 110 base pair region within the promoter of the human β-globin gene. The monomethyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of monomethyl-histone H3 (Lys27)-associated chromatin.
The ChIPAb+ Monomethyl-Histone H3 (Lys27) set includes the anti-monomethyl-histone H3 (Lys27) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 110 base pair region within the promoter of the human β-globin gene. The monomethyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of monomethyl-histone H3 (Lys27)-associated chromatin.
Specyficzność
Monomethyl-Histone H3 (Lys27)
Immunogen
The monomethyl-histone H3 (Lys27) antiserum is made against a synthetic 2X-branched peptide containing the sequence…ARmeKSA…in which meK corresponds to monomethyl-lysine 27 of human histone H3.
Zastosowanie
Chromatin Immunoprecipitation:
Successful immunoprecipitation of monomethyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter or primers amplifying the promoter of human β-globin, which is transcriptionally inactive in HeLa cells (Please see figures). Percent Input relative to standard curves for each qPCR primer set are shown, with immunoprecipitated DNA from control serum shown as (-) and monomethyl- histone H3 (Lys27) as (+).
Please refer to the EZ-Magna A ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western blot analysis and peptide inhibition:
HeLa Acid extract was resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-Monomethyl-Histone H3 (Lys27) (1:5000, Lane 1) or preincubated with 0.4 μM Histone H3 peptide with following modifications:
Lane 2: Linear non-modified
Lane 3: Branched monomethyl
Lane 4: Linear monomethyl
Lane 5: Branched dimethyl
Lane 6: Linear dimethyl
Lane 7: Branched trimethyl
Lane 8: Linear trimethyl
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Successful immunoprecipitation of monomethyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter or primers amplifying the promoter of human β-globin, which is transcriptionally inactive in HeLa cells (Please see figures). Percent Input relative to standard curves for each qPCR primer set are shown, with immunoprecipitated DNA from control serum shown as (-) and monomethyl- histone H3 (Lys27) as (+).
Please refer to the EZ-Magna A ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western blot analysis and peptide inhibition:
HeLa Acid extract was resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-Monomethyl-Histone H3 (Lys27) (1:5000, Lane 1) or preincubated with 0.4 μM Histone H3 peptide with following modifications:
Lane 2: Linear non-modified
Lane 3: Branched monomethyl
Lane 4: Linear monomethyl
Lane 5: Branched dimethyl
Lane 6: Linear dimethyl
Lane 7: Branched trimethyl
Lane 8: Linear trimethyl
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This ChIPAb+ Monomethyl-Histone H3 (Lys27) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Opakowanie
25 assays per kit, ~4μL per chromatin immunoprecipitation
Jakość
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 μL of either a normal rabbit antiserum or Anti-Monomethyl-Histone H3 (Lys27) serum and the Magna ChIP A Kit (Cat. #17-610).
Successful immunoprecipitation of monomethyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human β-globin promoter (Please see figures).
Please refer to the EZ-Magna A ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371).
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 μL of either a normal rabbit antiserum or Anti-Monomethyl-Histone H3 (Lys27) serum and the Magna ChIP A Kit (Cat. #17-610).
Successful immunoprecipitation of monomethyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human β-globin promoter (Please see figures).
Please refer to the EZ-Magna A ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371).
Opis wartości docelowych
17 kDa
Postać fizyczna
Anti-monomethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100 μL of antiserum containing 0.05% sodium azide.
Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% sodium azide.
ChIP Primers, β-Globin. One vial containing 75 μL of 5 μM of each primer specific for for human β-globin promoter.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% sodium azide.
ChIP Primers, β-Globin. One vial containing 75 μL of 5 μM of each primer specific for for human β-globin promoter.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt
Komentarz do analizy
Control
Included negative control antibody normal rabbit serum and control primers specific for human β-globin promoter.
Included negative control antibody normal rabbit serum and control primers specific for human β-globin promoter.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
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