16-222

Przeciwciało anty-fosfo-histonowe H3 (Ser10), klon 3H10, koniugat FITC

clone 3H10, Upstate^®, from mouse

• Synonim(y): Histon H3, rodzina 3A, H3S10P, histon H3 (fosfo S10), histon H3, rodzina 3A, histon H3, rodzina 3B, histon H3, rodzina 3B (H3.3B)
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• białko sprzężone: FITC conjugate
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 3H10, monoclonal
• reaktywność gatunkowa: human
• producent / nazwa handlowa: Upstate^®
• metody: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NP_003484
• numer dostępu UniProt: Q16695
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer10)
• informacje o genach: human ... HIST1H3F(8968)

Opis

Opis ogólny

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specyficzność

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

Immunogen

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Epitope: Ser10

Zastosowanie

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

Jakość

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.

Opis wartości docelowych

17 kDa

Postać fizyczna

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

Przechowywanie i stabilność

Stable for 1 year at from date of receipt.

Komentarz do analizy

Control
Mitotic HeLa cells (IF).

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2