16-193
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
clone JBW301, Upstate®, from mouse
Synonim(y):
H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
biotin conjugate
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
JBW301, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
phosphorylation (pSer139)
informacje o genach
human ... H2AX(3014)
Opis ogólny
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Specyficzność
A broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
Immunogen
Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Zastosowanie
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate is a Mouse Monoclonal Antibody for detection of phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone & has been validated in ICC & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Jakość
Routinely evaluated in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine (19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
Western Blot Analysis:
0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
Opis wartości docelowych
15 kDa
Postać fizyczna
Protein G Purified
Purified biotin conjugated mouse monoclonal IgG1 in buffer containing 70% storage buffer (0.02 M Phosphate buffer, pH7.6, 0.25 M NaCl, 0.1% sodium azide) and 30% glycerol
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Komentarz do analizy
Control
Staurosporine-treated Jurkat cell lysates.
Staurosporine-treated Jurkat cell lysates.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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