15-115
AMPK Pathway Explorer Antibody MiniPack
1 mg/mL, Upstate®
Synonim(y):
AMP-Activated Protein Kinase
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About This Item
Kod UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat (07-350SP, 07-363SP, 07-681SP), mouse (07-350SP), human (07-350SP, 07-363SP, 07-681SP)
producent / nazwa handlowa
Upstate®
stężenie
1 mg/mL
metody
activity assay: suitable (kinase and phosphatase)
western blot: suitable
numer dostępu UniProt
Warunki transportu
dry ice
informacje o genach
human ... PRKAA1(5562)
mouse ... Hsf3(245525)
Opis ogólny
Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
*See full size versions for corresponding references.
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
*See full size versions for corresponding references.
Specyficzność
07-681SP: AMPKα phosphorylated on Thr172.
07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).
07-350SP: AMPKα1
07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).
07-350SP: AMPKα1
07-681SP: Expected to cross-react with mouse based on 100% sequence homology.
Cross Reactivty
Varies. See individual Data Sheets
Varies. See individual Data Sheets
Immunogen
Epitope: 07-350SP and 07-363SP: Internal
Zastosowanie
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).
Opakowanie
Each vial is 30% the size of the parent catalog number
Komponenty
07-350SP Anti-AMPK α1; 60 µg
07-363SP Anti-AMPKα2; 60 µg
07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
07-363SP Anti-AMPKα2; 60 µg
07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
Jakość
07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.
07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.
Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.
07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.
07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.
Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.
07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Opis wartości docelowych
~63 kDa;
Postać fizyczna
3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)
Format: Purified
Protein A purified
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Komentarz do analizy
Control
For 07-681SP use HEK293 cells or rat liver AMPK.
For 07-363SP use Rat liver preparation.
For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
For 07-681SP use HEK293 cells or rat liver AMPK.
For 07-363SP use Rat liver preparation.
For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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10 - Combustible liquids
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