09-310

Anti-phospho-EGFR (Tyr1069) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Receptor tyrosine-protein kinase ErbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), pig (based on 100% sequence homology), horse (based on 100% sequence homology)
• metody: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NM_005228.3
• numer dostępu UniProt: P00533
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pTyr1069)
• informacje o genach: horse ... Egfr(100067755) ; human ... EGFR(1956) ; mouse ... Egfr(13649) ; pig ... Egfr(397070) ; rat ... Egf(25313)

Opis

Opis ogólny

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

Specyficzność

Reacts strongly and specifically with Tyr1069 phosphorylated EGFR

Immunogen

Epitope: Tyr1069 (cytoplasmic domain)

Tyrosine-phosphorylated epidermal growth factor receptor.

Zastosowanie

Immunofluroescent Immunocytochemistry:

Research Category
Apoptosis & Cancer

Signaling

Research Sub Category
Kinases & Phosphatases

This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).

Jakość

Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.

Opis wartości docelowych

~170 kDa

Powiązanie

Replaces: 04-339

Postać fizyczna

Antigen Affinity Purified

Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Komentarz do analizy

Control
Untreated and EGF treated A431 lysates

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable