07-706
Anti-WDR5 Antibody
Upstate®, from rabbit
Synonim(y):
BIG3, BMP2-induced 3-kb gene protein, Cilia and flagella associated protein 89, Set1c WD40 repeat protein homolog, SWD3, WD repeat-containing protein 5, WD-repeat protein 5
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... WDR5(11091)
Opis ogólny
WD repeat-containing protein 5 (also known as BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, WD-repeat protein 5, Set1c WD40 repeat protein homolog, cilia and flagella associated protein 89) is a nuclear protein encoded by the WDR5 (also known as BIG3, CFAP89, SWD3) gene in human. This protein contains 7 WD (Trp-Asp) repeats and belongs to the WD repeat protein family whose members are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeat-containing protein 5 is a component of several complexes that regulate histone methylation and acetylation levels, including ATAC (ADA2A-containing complex) complex, SET1 complex, MLL1/MLL, MLL2/3 (also named ASCOM complex), MLL4/WBP7, and NSL (non-specific lethal) complex.
Specyficzność
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Recognizes WDR5, MW 36 kDa. A related protein band of 43 kDa that was blocked by the immunizing peptide was also detected. Identification of WDR5 protein at 36 kDa was confirmed with siRNA knockdown by an independent laboratory.
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human WDR5.
Zastosowanie
Detect WDR5 using this Anti-WDR5 Antibody validated for use in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot: 0.05-0.25 μg/mL using HeLa nuclear extracts.
Jakość
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.
Opis wartości docelowych
~39 kDa observed
Postać fizyczna
Affinity purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
J Paggetti et al.
Oncogene, 29(36), 5019-5031 (2010-06-29)
MOZ and MLL, encoding a histone acetyltransferase (HAT) and a histone methyltransferase, respectively, are targets for recurrent chromosomal translocations found in acute myeloblastic or lymphoblastic leukemia. In MOZ (MOnocytic leukemia Zinc-finger protein)/CBP- or mixed lineage leukemia (MLL)-rearranged leukemias, abnormal levels
B Bidon et al.
Nature communications, 9(1), 2610-2610 (2018-07-06)
The DNA damage sensor XPC is involved in nucleotide excision repair. Here we show that in the absence of damage, XPC co-localizes with RNA polymerase II (Pol II) and active post-translational histone modifications marks on a subset of class II
Yul W Yang et al.
eLife, 3, e02046-e02046 (2014-02-14)
The WDR5 subunit of the MLL complex enforces active chromatin and can bind RNA; the relationship between these two activities is unclear. Here we identify a RNA binding pocket on WDR5, and discover a WDR5 mutant (F266A) that selectively abrogates
Ruosi Yao et al.
Oncology reports, 40(5), 3069-3077 (2018-09-19)
Accumulating evidence has revealed that the methylation of lysines on nonhistones by histone lysine methyltransferases (HMTs) is crucial for regulating tumo-rigenesis and metastasis. However, whether the methy-lation of lysines on HMT complex components occurs and has functions in cancer progression
Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.
Wu, M; Wang, PF; Lee, JS; Martin-Brown, S; Florens, L; Washburn, M; Shilatifard, A
Molecular and cellular biology null
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