07-329-AF488

Anti-acetyl-Histone H4 (Lys16) Antibody, Alexa Fluor^™ 488

from rabbit, ALEXA FLUOR^™ 488

• Synonim(y): Histone H4, acetyl-Histone H4 (Lys16), AlexaFluor^™ 488
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• białko sprzężone: ALEXA FLUOR^™ 488
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: mouse, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology)
• metody: immunocytochemistry: suitable
• numer dostępu NCBI: NP_009563.1
• numer dostępu UniProt: P02309
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: acetylation (Lys16)
• informacje o genach: human ... H4C1(8359) ; mouse ... H4C1(326619)

Opis

Opis ogólny

Histone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specyficzność

Broad species cross-reactivity is expected.

H4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Immunogen

Epitope: H4K16ac

Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.

Zastosowanie

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.

This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).

Jakość

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.

Opis wartości docelowych

~10 kDa observed

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Informacje prawne

ALEXA FLUOR is a trademark of Life Technologies

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable