07-2129

Anti-phospho-Abi-1 (Ser225) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• klon: polyclonal
• application: WB
• reaktywność gatunkowa: monkey, human
• metody: western blot: suitable

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: monkey, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_001012768
• numer dostępu UniProt: Q8IZP0
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer225)
• informacje o genach: human ... ABI1(10006)

Opis

Opis ogólny

Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.

Specyficzność

This antibody recognizes Abi-1 phosphorylated at Ser225.

Immunogen

Epitope: Phosphorylated Ser225

KLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.

Zastosowanie

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).

Jakość

Evaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.

Opis wartości docelowych

~60 kDa observed.
55 kDa calculated.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
EGF-treated COS cells untreated and treated with lambda phosphatase.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable