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Wszystkie zdjęcia(6)

Key Documents

06-536

Sigma-Aldrich

Anti-Bak Antibody, NT

Upstate®, from rabbit

Synonim(y):

Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like 7 protein, pro-apoptotic protein BAK

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

reaktywność gatunkowa

mouse, rat, human

producent / nazwa handlowa

Upstate®

metody

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... BAK1(578)

Opis ogólny

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.

Specyficzność

Specific for Bak, MW ~28 kDa.

Immunogen

Epitope: N-terminus
Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.

Zastosowanie

Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB.
Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition

Jakość

Evaluated by Western Blot on HEK293 lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.

Opis wartości docelowych

28 kDa

Powiązanie

Replaces: 04-433

Postać fizyczna

Format: Purified
Protein A purified
Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid.

Przechowywanie i stabilność

Stable for 1 year at -20ºC from date of receipt.

Komentarz do analizy

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Stephanie Einsele-Scholz et al.
Journal of cell science, 129(11), 2213-2223 (2016-04-15)
The pro-apoptotic multidomain Bcl-2 proteins Bax and Bak (also known as BAK1) are considered the gatekeepers of the intrinsic pathway of apoptosis by triggering the mitochondrial release of cytochrome c The role of the third Bax- and Bak-homologous multidomain protein
Bim is the key mediator of glucocorticoid-induced apoptosis and of its potentiation by rapamycin in human myeloma cells.
Lopez-Royuela N, Balsas P, Galan-Malo P, Anel A, Marzo I, Naval J
Biochimica et Biophysica Acta null
Bcl-xES, a BH4- and BH2-containing antiapoptotic protein, delays Bax oligomer formation and binds Apaf-1, blocking procaspase-9 activation.
Schmitt, E; Paquet, C; Beauchemin, M; Bertrand, R
Oncogene null
Evidence for involvement of BH3-only proapoptotic members in adenovirus-induced apoptosis.
Subramanian, T; Vijayalingam, S; Lomonosova, E; Zhao, LJ; Chinnadurai, G
Journal of virology null
Molecular regulation of DNA damage-induced apoptosis in neurons of cerebral cortex.
Martin, LJ; Liu, Z; Pipino, J; Chestnut, B; Landek, MA
Cerebral Cortex (1991)

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