06-1433

Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Basic fibroblast growth factor receptor 1, FGFR-1, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, Proto-oncogene c-Fgr, CD331
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• opakowanie: antibody small pack of 25 μg
• metody: cell based assay: suitable; western blot: suitable
• numer dostępu NCBI: NP_075598
• numer dostępu UniProt: P11362
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: phosphorylation (pTyr653/pTyr654)
• informacje o genach: human ... FGFR1(2260)

Opis

Opis ogólny

FGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation.

Specyficzność

This antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654.

Immunogen

Epitope: Phosphorylated Tyr653/Tyr654

KLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654.

Zastosowanie

Detect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay.

Peptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Jakość

Evaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.

Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.

Opis wartości docelowych

~145 kDa observed.
FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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