05-623
Anti-RNA polymerase II Antibody, clone CTD4H8
clone CTD4H8, Upstate®, from mouse
Synonim(y):
Anti-NEDHIB, Anti-POLR2, Anti-POLRA, Anti-RPB1, Anti-RPBh1, Anti-RPO2, Anti-RPOL2, Anti-RpIILS, Anti-hRPB220, Anti-hsRPB1
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
CTD4H8, monoclonal
reaktywność gatunkowa
Saccharomyces cerevisiae, human, mouse, rat
opakowanie
antibody small pack of 25 μg
producent / nazwa handlowa
Upstate®
metody
ChIP: suitable (ChIP-seq)
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)
Opis ogólny
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Specyficzność
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Immunogen
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Zastosowanie
Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Jakość
Routinely evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Opis wartości docelowych
210-220 kDa
Postać fizyczna
Format: Purified
Protein G Chromatography
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Komentarz do analizy
Control
A431 nuclear extract.
A431 nuclear extract.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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