04-841
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
culture supernatant, clone A9W4, from rabbit
Synonim(y):
RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
A9W4, monoclonal
application:
IP
WB
WB
reaktywność gatunkowa:
rat, human, mouse, bovine
metody:
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
4
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
A9W4, monoclonal
reaktywność gatunkowa
rat, human, mouse, bovine
metody
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
rat ... Rock2(25537)
Opis ogólny
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
Specyficzność
Predicted to cross-react with mouse and bovine based on sequence homology.
Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
Immunogen
6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
Zastosowanie
Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Jakość
Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Opis wartości docelowych
150 kDa
Powiązanie
Replaces: 05-841
Postać fizyczna
Cultured supernatant containing 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
RIPA lysates from PC-12 cells
RIPA lysates from PC-12 cells
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
T Leung et al.
Molecular and cellular biology, 16(10), 5313-5327 (1996-10-01)
The GTPase RhoA has been implicated in various cellular activities, including the formation of stress fibers, motility, and cytokinesis. We recently reported on a p150 serine/threonine kinase (termed ROK alpha) binding RhoA only in its active GTP-bound state and on
S Vincent et al.
Molecular and cellular biology, 17(4), 2247-2256 (1997-04-01)
The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly with protein kinases, which are likely to serve as downstream effector
Ines Jeric et al.
Nature communications, 7, 13781-13781 (2016-12-22)
Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths, but its molecular heterogeneity hampers the design of targeted therapies. Currently, the only therapeutic option for advanced HCC is Sorafenib, an inhibitor whose targets include RAF. Unexpectedly, RAF1 expression is
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