04-079
Anti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal
culture supernatant, Upstate®
Synonim(y):
H4K20me3, Histone H4 (tri methyl K20)
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klon:
monoclonal
application:
ChIP
DB
WB
DB
WB
reaktywność gatunkowa:
vertebrates, human
metody:
ChIP: suitable
dot blot: suitable
western blot: suitable
dot blot: suitable
western blot: suitable
citations:
4
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
monoclonal
reaktywność gatunkowa
vertebrates, human
producent / nazwa handlowa
Upstate®
metody
ChIP: suitable
dot blot: suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
trimethylation (Lys20)
informacje o genach
human ... H4C1(8359)
Opis ogólny
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
Specyficzność
Recognizes Histone H4 when trimethylated on Lys20.
Immunogen
Peptide corresponding to human Histone H4 containing the sequence [HRmethKVL] on which lysine 20 is trimethylated.
Zastosowanie
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-079, Anti-trimethyl Histone H4 (Lys20) (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-079, Anti-trimethyl Histone H4 (Lys20) (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Detect trimethyl-Histone H4 (Lys20) using this Anti-trimethyl-Histone H4 (Lys20) Antibody, rabbit demontrated performance in WB, ChIP & DB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Jakość
Routinely evaluated by immunoblot.
Opis wartości docelowych
11kDa
Postać fizyczna
100 μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide
Przechowywanie i stabilność
2 years at -20°C from date of shipment
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Volodymyr P Tryndyak et al.
Cancer biology & therapy, 5(1), 65-70 (2005-12-03)
Cancer cells are characterized by epigenetic dysregulation, including global genome hypomethylation, regional hypo- and hypermethylation, altered histone modifications, and disturbed genomic imprinting. Despite the long-established fact that global DNA hypomethylation is a common feature of tumors, very little is known
Dmitry Karachentsev et al.
Developmental biology, 304(1), 46-52 (2007-01-19)
Methylation of specific amino acids in histone tails is responsible for packaging DNA into condensed, repressed chromatin, and into open chromatin that is accessible to the transcription machinery. Monomethylation and trimethylation of histone H4-lysine 20 (H4-K20) control the formation of
Histone trimethylation and the maintenance of transcriptional ON and OFF states by trxG and PcG proteins.
Papp, B; Muller, J
Genes & Development null
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