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WTA1
TransPlex® Whole Transcriptome Amplification Kit
DNA polymerase separate.
Synonim(y):
Zestaw do amplifikacji transkryptomu
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About This Item
Kod UNSPSC:
41121800
NACRES:
NA.55
Polecane produkty
Poziom jakości
metody
whole genome amplification: suitable
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Zastosowanie
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.
Cechy i korzyści
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Zasada
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Informacje prawne
TransPlex is a registered trademark of Rubicon Genomics, Inc.
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produkt powiązany
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
Protokoły
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
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