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P4030

Sigma-Aldrich

Phloxine B

Dye content ≥80 %, certified by the Biological Stain Commission

Synonym(s):

2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt, Acid Red 92, Cyanosine, D&C;Red No. 28, Eosin 10B

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About This Item

Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
18472-87-2
Molecular Weight:
829.63
Colour Index Number:
45410
Beilstein:
3887100
EC Number:
242-355-6
MDL number:
MFCD00070627
UNSPSC Code:
12171500
PubChem Substance ID:
24898486
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥80%

color

dark red

solubility

H2O: 1 mg/mL

εmax

1150-1300 at 546-550 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

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General description

Phloxine B is part of the xanthylium class of dyes. The stain is mostly used to determine cell ploidy and temperature sensitivity of Schizosaccharomyces pombe. Solid media is colored a vivid pink.

Application

Phloxine B has been used as a component of yeast extract peptone dextrose (YEPD) to grow Schizosaccharomyces pombe.

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Kreyberg′s method for keratin and mucus.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM7086
MKCJ0760
MKCF5408
MKBX6395V
MKBX6581V

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The Small Subunit Processome Is Required for Cell Cycle Progression at G1
Kara A. Bernstein and Susan J. Baserga
Molecular Biology of the Cell (2004)
Basic methods for fission yeast
Susan L
Yeast (2006)
Ander M de Lecea et al.
Rapid communications in mass spectrometry : RCM, 25(13), 1853-1861 (2011-06-04)
Stable isotope measurements are an important tool for ecosystem trophic linkage studies. Ideally, fresh samples should be used for isotopic analysis, but in many cases organisms must be preserved and analysed later. In some cases dyes must be used to
Hang Qi et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 50(6), 1841-1847 (2012-03-24)
We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun-N-terminal kinase (JNK) in an oxidative
Advances in Food and Nutrition Research, 61 (2010)
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Protocols

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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