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Key Documents

GE29-0486-09

GSTrap 4B

Cytiva 29-0486-09, pack of 1 mL

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

glutathione

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0486-09

availability

not available in North America

parameter

42 psi (H2O at 25°C)

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C)

General description

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

Application

Tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System.

Features and Benefits

  • High binding capacity.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

GSTrap is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

Protocolos

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico