GE17-5319-01
HisTrap™ Fast Flow
Cytiva 17-5319-01, pack of 5 × 1 mL
About This Item
Productos recomendados
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5319-01
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
Categorías relacionadas
General description
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Features and Benefits
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Artículos
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
Protocolos
This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
Contenido relacionado
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
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