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G4510

Millipore

Glutathione−Agarose

lyophilized powder

Sinónimos:

Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose

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About This Item

MDL number:
MFCD00131200
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

analyte chemical class(es)

proteins (GST)

extent of labeling

≥10 μmol per mL gel

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

matrix

4% cross-linked beaded agarose

matrix activation

epoxy

matrix attachment

sulfur

matrix spacer

12 atoms (10 carbon)

storage temp.

−20°C

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General description

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Application

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Biochem/physiol Actions

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Features and Benefits

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Quantity

One g powder swells to ≥10 ml of gel

Physical form

Lyophilized powder stabilized with lactose

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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L-Glutatión reducido ≥98.0%

Sigma-Aldrich

G4251

L-Glutatión reducido

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Wenrong Tan et al.
PLoS genetics, 14(4), e1007336-e1007336 (2018-04-17)
Drought is a major threat to plant growth and crop productivity. The phytohormone abscisic acid (ABA) plays a critical role in plant response to drought stress. Although ABA signaling-mediated drought tolerance has been widely investigated in Arabidopsis thaliana, the feedback
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation
P G Board et al.
The Journal of biological chemistry, 275(32), 24798-24806 (2000-04-28)
A new class of glutathione transferases has been discovered by analysis of the expressed sequence tag data base and sequence alignment. Glutathione S-transferases (GSTs) of the new class, named Omega, exist in several mammalian species and Caenorhabditis elegans. In humans
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Hui Li et al.
Nature communications, 9(1), 2573-2573 (2018-07-04)
The dynamic maintenance of polar domains in the plasma membrane (PM) is critical for many fundamental processes, e.g., polar cell growth and growth guidance but remains poorly characterized. Rapid tip growth of Arabidopsis pollen tubes requires dynamic distribution of active
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