추천 제품
설명
AF-Chelate-650M
제품 라인
TOYOPEARL®
양식
liquid
포장
bottle of 25 mL
제조업체/상표
Tosoh TOYOPEARL® AF, 14475
라벨링 범위
25-45 μeq/mL ligand density
파라미터
3 bar max. pressure
기술
HPLC: suitable
Matrix
hydroxylated methacrylic polymer
기질 활성군
IDA phase, iminodiacetic acid
입자 크기
65 μm
작동 pH
2-12
분리 기술
affinity
저장 온도
2-8°C
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일반 설명
Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.
애플리케이션
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
규격
Clean in place with 0.5 M NaOH or 0.1 M HCl.
물리적 형태
Shipped in 20% (v/v) ethanol.
법적 정보
Toyopearl is a registered trademark of Tosoh Corporation
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
WGK
WGK 1
Flash Point (°F)
103.1 °F
Flash Point (°C)
39.5 °C
가장 최신 버전 중 하나를 선택하세요:
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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