ZMS1221

Anti-LAMP2 Antibody, clone H4B4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): CD107 antigen-like family member B, CD107b, LAMP-2, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: H4B4, recombinant monoclonal
• 설명: recombinant, expressed in HEK 293 cells
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 44.9 kDa; observed mol wt ~105 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 25
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_001116078
• UniProt 수납 번호: P13473
• 환경친화적 대안 카테고리: Aligned
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 유전자 정보: human ... LAMP2(3920)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

특이성

Clone H4B4 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects LAMP-2.

면역원

Human adherent peripheral blood cells.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected LAMP2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected LAMP2 in lysate from U937 cells and 1:1,000 dilution in lysate from Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LAMP2 in Human liver tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected LAMP2 in one million U937 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Lysosome-associated membrane glycoprotein 2 (UniProt: P13473; also known as LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, CD107b) is encoded by the LAMP2 gene (Gene ID: 3920) in human. LAMP-2 is a single-pass type I, heavily glycosylated, membrane protein found in plasma membrane, endosomes, and lysosomes. It is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 29-375), a transmembrane domain (aa 376-399), and a short cytoplasmic tail (aa 400-410). It is an important component of lysosomal membranes and is involved in both autophagy and lysosomal membrane permeabilization. LAMP-2 plays an important role in chaperone-mediated autophagy. It is required for the fusion of autophagosomes with lysosomes during autophagy. It also plays a role in lysosomal protein degradation in response to starvation. It functions by binding target proteins, such as GAPDH and MLLT11, and targets them for lysosomal degradation. In cancer cells, chronic acidosis-induced upregulation of LAMP-2 has been reported that protects these cells from acid-induced hydrolysis and promotes their survival via chaperone-mediated autophagy. Loss of LAMP-2 is reported to aggravate oxidative stress through the obstruction of reactive oxygen species clearance. Three different isoforms of LAMP-2 have been described that are produced by alternative splicing. Mutations in LAMP2 gene have been linked to Danon disease that is characterized by cardiomyopathy, vascular myopathy, and mental retardation. It is often associated with accumulation of glycogen in muscle and lysosomes. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cui, L., et al. (2020). Front. Cell Dev. Biol. 8; 31; Qin, X., et al. (2017). Biochem. Biophys. Res. Commun. 488(1); 177-181; Cuervo, AM., and Dice, JF. (2000). J. Cell Sci. 113(24); 4441-4450).

물리적 형태

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable