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Merck
모든 사진(2)

주요 문서

T9650

Sigma-Aldrich

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

동의어(들):

TAE buffer

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About This Item

MDL number:
UNSPSC 코드:
41105319
PubChem Substance ID:
NACRES:
NA.25

Grade

for molecular biology

Quality Level

무균

non-sterile; 0.2 μm filtered

제품 라인

BioReagent

양식

solution

적합성

suitable for gel electrophoresis (after dilution to working concentration)

외래 활성

Protease, none detected
RNAse, none detected

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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애플리케이션

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

기타 정보

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

제조 메모

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

STOT RE 2 Inhalation

표적 기관

Respiratory Tract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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프로토콜

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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