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Merck
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주요 문서

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93337

Sigma-Aldrich

Trizma® acetate

BioUltra, ≥99.0% (NT)

동의어(들):

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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About This Item

Linear Formula:
NH2C(CH2OH)3 · CH3COOH
CAS Number:
6850-28-8
Molecular Weight:
181.19
Beilstein:
3702918
EC Number:
229-939-6
MDL number:
MFCD00038951
UNSPSC 코드:
12161700
PubChem Substance ID:
57653535
NACRES:
NA.25

제품 라인

BioUltra

분석

≥99.0% (NT)

양식

crystalline powder

불순물

insoluble matter, passes filter test

무기 잔류물

≤0.2%

손실

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

음이온 미량물

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

양이온 미량물

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV 흡수

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

SMILES string

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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애플리케이션

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

기타 정보

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferasecitation; Assay of glutamate bindingcitation
Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
BCCG6097
BCCG3737
BCCG1638
BCCF9323
BCCF1884

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문서 라이브러리 방문

Chi-Lin Li et al.
The Analyst, 137(22), 5222-5228 (2012-10-04)
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase.
W W Nichols et al.
Analytical biochemistry, 114(2), 396-397 (1981-07-01)
M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
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