T4376

N-p-Tosyl-L-phenylalanine chloromethyl ketone

powder, ≥97% (TLC)

• 동의어(들): (S)-1-Chloro-3-tosylamido-4-phenyl-2-butanone, (S)-1-Chloro-4-phenyl-3-tosylamido-2-butanone, TPCK, Tosyl-L-phenylalanyl-chloromethane
• 실험식(Hill 표기법): C₁₇H₁₈ClNO₃S
• CAS Number: 402-71-1
• Molecular Weight: 351.85
• Beilstein: 2895215
• EC Number: 206-954-6
• MDL number: MFCD00000935
• UNSPSC 코드: 12352202
• PubChem Substance ID: 24278737
• NACRES: NA.77

속성

• product name: N-p-Tosyl-L-phenylalanine chloromethyl ketone, ≥97% (TLC), powder
• 생물학적 소스: synthetic
• Quality Level: 200
• 분석: ≥97% (TLC)
• 형태: powder
• 효능: 10-100 μM effective concentration
• 색상: white
• mp: 106-108 °C (lit.)
• solubility: DMSO: >10 mg/mL (stable for several months at 4°C.); ethanol: 20 mg/mL
• 저장 온도: −20°C
• SMILES string: Cc1ccc(cc1)S(=O)(=O)N[C@@H](Cc2ccccc2)C(=O)CCl
• InChI: 1S/C17H18ClNO3S/c1-13-7-9-15(10-8-13)23(21,22)19-16(17(20)12-18)11-14-5-3-2-4-6-14/h2-10,16,19H,11-12H2,1H3/t16-/m0/s1
• InChI key: MQUQNUAYKLCRME-INIZCTEOSA-N
• 유전자 정보: human ... NFKB1(4790) , NKAP(79576) , NKRF(55922) , NOS2(4843) , PTGS2(5743)

설명

일반 설명

stable for several months at 4°C. Stock solutions (10 mM in methanol or ethanol) are stable for several months at 4°C.

애플리케이션

N-p-Tosyl-L-phenylalanine chloromethyl ketone has been used:

• to investigate whether transcription factor NFκB is involved in renalase expression
• to test the role of NF-κB activation
• to study its inhibitory effect on chymotrypsin

Irreversible inhibitor of certain serine proteases; used to inhibit chymotrypsin in preparations of trypsin.

생화학적/생리학적 작용

Blocks the LPS- or cytokine-induced activation of nuclear factor κB (NFκB), which, in turn, blocks the induction of iNOS and COX-2 transcription. Blocks activation of pp70^s6k by all mitogens. Blocks apoptosis cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. Also blocks apoptosis initiated by Taxol (in MCF-7 human breast carcinoma cells).

N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NFκB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. Also blocks apoptosis initiated by Taxol (in MCF-7 human breast carcinoma cells). TPCK recognises the phenylalanine residue in chymotrypsin′s and prevents its function.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

주의사항

Shelf-life is at least two years when stored desiccated at -20 °C.

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H318,H335
• 예방조치 성명서: P280 - P302 + P352 - P305 + P351 + P338 + P310
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves