T4019

Trypsin−Agarose

buffered aqueous suspension, from bovine pancreas trypsin

• MDL number: MFCD00082094
• UNSPSC 코드: 12352204
• eCl@ss: 42020142
• NACRES: NA.54

속성

• 생물학적 소스: bovine pancreas (trypsin)
• Quality Level: 200
• 형태: buffered aqueous suspension
• 농도: ≥15 units/mL (packed gel)
• 라벨링 범위: ≥15 units per mL (packed gel)
• 기질: beaded agarose
• 저장 온도: 2-8°C

설명

일반 설명

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

애플리케이션

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

단위 정의

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

물리적 형태

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

제조 메모

TPCK-treated

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable