SRP6557

Thrombin Active from human plasma

≥95% (SDS-PAGE), recombinant, lyophilized

• 동의어(들): Activated Factor IIa
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• product name: Thrombin Active from human plasma, ≥95% (SDS-PAGE)
• 생물학적 소스: human
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized
• 효능: >3000 units/mg
• 분자량: 37 kDa
• 포장: pkg of 1000 units; pkg of 250 units
• UniProt 수납 번호: P00734
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... F2(2147)

설명

일반 설명

Thrombin enzyme (activated factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. It is primarily associated with blood clotting.

생화학적/생리학적 작용

Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. It also induces inflammation. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

물리적 형태

Lyophilized from a buffer composed of 20 mM Bis-Tris, 150 mM NaCl and 0.1% PEG 8000, pH 6.5.

재구성

A working stock solution can be prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25 mM HEPES, 150 mM NaCl, 0.1% PEG 8000, pH 7.4). Further dilution should be made in buffer containing a suitable stabilizing agent such as 0.1%-1% Prionex, BSA, or PEG.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H319,H334
• 예방조치 성명서: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3