SRP6556

Thrombin Active (High Activity) from bovine plasma

≥98% (SDS-PAGE), recombinant, lyophilized

• 동의어(들): Activated Factor IIa
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 제품명: Thrombin Active (High Activity) from bovine plasma, ≥98% (SDS-PAGE)
• 생물학적 소스: bovine plasma
• 분석: ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: >1500 units/mg
• 분자량: 37 kDa
• 포장: pkg of 10,000 units; pkg of 100,000 units; pkg of 1000 units
• UniProt 수납 번호: P00735
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: bovine ... F2(280685)

설명

일반 설명

Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.

생화학적/생리학적 작용

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells. It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.

물리적 형태

Sterile filtered and lyophilized with Mannitol and Sodium Chloride.

재구성

Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable