추천 제품
product name
Thrombin Active (High Activity) from bovine plasma, ≥98% (SDS-PAGE)
생물학적 소스
bovine plasma
분석
≥98% (SDS-PAGE)
형태
lyophilized
효능
>1500 units/mg
분자량
37 kDa
포장
pkg of 10,000 units
pkg of 100,000 units
pkg of 1000 units
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
bovine ... F2(280685)
일반 설명
Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.
생화학적/생리학적 작용
Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells. It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.
물리적 형태
Sterile filtered and lyophilized with Mannitol and Sodium Chloride.
재구성
Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
The CUG codon is decoded in vivo as serine and not leucine in Candida albicans.
Santos MA and Tuite MF
Nucleic Acids Research, 23, 1481-1486 (1995)
Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model.
Bruce AC and Peirce SM
Microcirculation (New York, N.Y. : 1994), 19, 143-154 (2012)
Effects of coagulation factor deficiency on plasma coagulation kinetics determined via thrombelastography: critical roles of fibrinogen and factors II, VII, X and XII.
Nielsen VG, et al.
Acta Anaesthesiologica Scandinavica, 49, 222-231 (2005)
A dual thrombin receptor system for platelet activation.
Kahn ML, et al.
Nature, 394, 690-694 (1998)
Biophysical investigation of GpIbalpha binding to thrombin anion binding exosite II.
Sabo TM and Maurer MC
Biochemistry, 48, 7110-7122 (2009)
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