SRP4902

Adiponectin from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 동의어(들): ACDC, APM-1, Acrp30, AdipoQ, GBP-28
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 양식: lyophilized
• 분자량: ~30 kDa
• 포장: pkg of 25 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 불순물: endotoxin, tested
• NCBI 수납 번호: Q60994
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... Adipoq(11450)

설명

일반 설명

Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active. gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1.

애플리케이션

Adiponectin from mouse has been used for the treatment of 3T3-L1 cells, along with TNFα (tumor necrosis factor), to determine TNFα inhibition of adiponectin activities by examining lipid accumulation and glucose uptake. It has also been used for the pretreatment of TM3 cells, to study its influence on attenuation of proinflammatory cytokine expression in TM3 cells upon treatment with TNF-α or IL-1β (interleukin).

생화학적/생리학적 작용

In obese mice and humans, adiponectin mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake. This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα(estrogen receptor alpha) status of cells are key in determining BRCA (breast cancer) cell growth.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 μg of bovine serum albumin (BSA) per 1 μg of adiponectin.

재구성

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable